Coding

Part:BBa_K4759019:Design

Designed by: Mengrui Tao   Group: iGEM23_Jiangnan-China   (2023-10-02)


TF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 505
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 73
    Illegal SapI.rc site found at 721
    Illegal SapI.rc site found at 834
    Illegal SapI.rc site found at 1090


Design Notes

Fusion expression with Olep


Source

TF is derived from E. coli and is highly efficiently expressed in E. coli with good soluble effect compared with tags from other biological sources.

References